| 產(chǎn)品名稱(chēng) |
human c-cbl |
| 商品貨號(hào) |
B190333 |
| Designations |
human c-cbl |
| GenBank Number |
X57110
|
| Species |
Homo sapiens, human |
| Depositors |
WY Langdon |
| Vector |
Construct size (kb): 5.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC
Intact vector size: 2.700
Type of vector: plasmid
Vector end: SmaI
Vector end: PstI
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CBL
Other gene symbols: CBL2
Genomic copy number: unique
Gene name: Cas-Br-M (murine) ecotropic retroviral transforming
sequence
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11 q23.3-qter
Tissue: HUT-78 and CCRF-CEM cell lines
Type of DNA: cDNA
Insert 5' end: ApaI
Insert 3' end: PstI
Insert size (kb): 2.9
Cross references: DNA Seq. Acc.: X57110
Nucleotides 1-2886 of the insert correspond to
nucleotides 1-2886 of X57110. Insert lengths(kb): 2.900000095367432 Tissue: HUT-78 and CCRF-CEM cell lines Gene product: Cas-Br-M (murine) ecotropic retroviral transforming sequence(Cas NS-1 viral (v-cbl) oncogene homolog) [CBL] |
| Insert Size (kb) |
2.900 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--5.6; EcoRI--3.2, 2.4; PstI--2.9, 2.7; SacI--3.0, 1.5, 1.3. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.17, 1.5; EcoRI--0.58; HindIII--0.72, 1.62, 2.78; BglII--1.77. |
| References |
Blake TJ, et al. The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif. Oncogene 6: 653-657, 1991. PubMed: 2030914
Wallace Y Langdon, personal communication
|
