contains easily purifiable cassette(s) for construction pMB1 ori - rop cassette
vector for other uses pMB1 ori - rop cassette
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--2.15; EcoRI--2.15; NotI--1.4, 0.85.
Helper plasmid with a NotI cassette containing a replication origin from pBR322.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348
