| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.5, 4.2, 3.7 and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 7.421, 0.401 (vector); 4.193, 3.761, 3.064, 2.750, 2.416, 2.210, 2.104, 1.738, 1.738, 1.676, 1.276, 1.139, 0.961, 0.879, 0.824, 0.821, 0.694, 0.694, 0.642, 0.499, 0.469, 0.394. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 382002-419951 of the sequence of chromosome VIII. |
| References |
Gimeno CJ, Fink GR. Induction of pseudohyphal growth by over expression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol. Cell. Biol. 14: 2100-2112, 1994. PubMed: 8114741
Linda Riles, personal communication
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