| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--16.0, 14.0, 7.8, 4.2, 2.2; EcoRI--13.0, 7.8, 7.6, 5.6, 4.3, 4.0, 1.3; BglII--14.0, 1.3, 1.9, 1.7, 1.5, 1.5, 1.2, 1.1 + smaller; NotI--40.0, 8.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Enzyme(s) not detecting polymorphism: MspI, PstI. |
| References |
Julier C, et al. A detailed genetic map of the long arm of chromosome 11. Genomics 7: 335-345, 1990. PubMed: 1973139
Julier C, et al. Isolation and mapping of a polymorphic DNA sequence (CJ52.193) on chromosome 11 [D11S384]. Nucleic Acids Res. 17: 9501, 1989. PubMed: 2573889
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