This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
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The Y. lipolytica portion of the plasmid contains the following restriction sites: EcoRI (5); SphI (1); SalI (2); AvaI (8); KpnI (1); EcoRI (4); XhoI (2), BglII (2). It lacks HindIII, ClaI, BamHI, and NruI sites.
Replicates autonomously in E. coli and integrates but does not replicate autonomously in Y. lipolytica.
Constructed by a inserting a 6.6 kb Sau3AI fragment (partial) containing LEU2 into the BamHI site of pBR322. The gene is contained in an EcoRI fragment (partial) of approximately 2.4 kb.
Media
ATCC® Medium 1245: YEPD
Growth Conditions
Temperature: 25.0°C
References
Davidow LS, DeZeeuw JR. Process for transformation of Yarrowia lipolytica. US Patent 4,880,741 dated Nov 14 1989
