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H5.46 [546]
H5.46 [546]
規(guī)格:
貨期:
編號:B190760
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 H5.46 [546]
商品貨號 B190760
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Its neutralizing activity confers protection in C57BL/6J mice (sIgM-/-) that were deficient in the production of secreted IgM but capable of expressing surface IgM [PubMed: 14662909].
The antibody reacts with a virus specific epitope, the E protein.
Storage Conditions liquid nitrogen vapor phase
Genes Expressed
immunoglobulin; monoclonal antibody; against West Nile Virus
Cellular Products
immunoglobulin; monoclonal antibody; against West Nile Virus
Comments
The antibody reacts with a virus specific epitope, the E protein. Its neutralizing activity confers protection in C57BL/6J mice (sIgM-/-) that were deficient in the production of secreted IgM but capable of expressing surface IgM [PubMed: 14662909].
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with: 10% NCTC 109 1% OPI (0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin) 15% fetal bovine serum
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 2 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype mouse IgM kappa
Name of Depositor RB Tesh
Deposited As mouse (B cell); mouse (myeloma)
References

Gould EA, et al. Antigencity of flaviviruses. Arch. Virol. Suppl. 1: 137-152, 1990.

The antibody reacts with a virus specific epitope.

Diamond MS, et al. A critical role for induced IgM in the protection against West Nile virus infection. J. Exp. Med. 198: 1853-1862, 2003. PubMed: 14662909

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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