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Construct size (kb): 3.880000114440918

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: GNAI2*
Gene name: guanine nucleotide binding protein (G protein), alpha
inhibiting activity polypeptide 2
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random-primed
(Stratagene 936205)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.98
Cross references: DNA Seq. Acc.: M78045; EST: EST01632; Primer: M13 Forward;
THC id: THC122443

Insert lengths(kb): 0.9800000190734863

Tissue: hippocampus, female, 2 years, oligo-dT and random-primed (Stratagene 936205)

Gene product: guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 [GNAI2*]

Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 0.98; PstI--3.9; BamHI--3.9.

EST01632 matches GenBank accession HUMGI2A2 with 97.42% identity over the sequence range: 233-1.