| 產品名稱 |
pVE3 [MB4597] |
| 商品貨號 |
B191393 |
| Designations |
pVE3 [MB4597] |
| Depositors |
Merck & Co., Inc., T MacNeil, Merck & Co., Inc. |
| U.S. Patent |
|
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli RR1; K-12 RR1 |
| Vector Information |
Size (kb): 17.2999897003173800 Vector: pVE3 (plasmid) Construction: pVE1, pIJ39 Marker(s):ampR,tsrR Construct size (kb): 17.29998970031738 Features: marker(s): ampR, tsrR
replicon: pVE1, pMB1 |
| Applications |
shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--10.1, 5.9; HindIII--10.1, 5.9; BamHI--10.5, 2.8, 1.7. A shuttle vector capable of replication in Escherichia coli and several Streptomyces species including lividans, avermitilis, and venezuelae. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
MacNeil T, Gibbons PH. rDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes. US Patent 4,703,009 dated Oct 27 1987
MacNeil DJ. Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612, 1988. PubMed: 3056907
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
