| 產品名稱 |
pB43BSP |
| 商品貨號 |
B192337 |
| Designations |
pB43BSP |
| Species |
Beet necrotic yellow vein virus |
| Depositors |
KE Richards |
| Vector |
Construct size (kb): 3.849999904632568 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.6399999856948853 Gene product: genome, partial |
| Insert Size (kb) |
0.640 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): PvuII--2.7, 0.9; HindIII--3.8; EcoRI--3.3, 0.5. To synthesize antisense RNA in vitro, cut with HindIII and transcribe from vector T7 promoter. The insert is nt 35-680 of RNA-4 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious. |
| Classification |
Furovirus |
| References |
Bouzoubaa S, et al. Nucleotide sequence analysis of RNA-3 and RNA-4 of beet necrotic yellow vein virus, isolates F2 and G1. J. Gen. Virol. 66: 1553-1564, 1985.
Ken E Richards, personal communication
|
