| Vector |
Construct size (kb): 7.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCMV5
Intact vector size: 4.700
Type of vector: phagemid
Vector end: EcoRI
Vector end: PstI
Cloning sites: EcoRI BamHI HindIII
Polylinker sites:
Host range: mammalian cells; Escherichia coli
Features (with orientation and position when available):
promoter: CMV, ->
terminator: hGH, ->
replicon: SV40
replicon: pMB1
marker(s): ampR, <-
replicon: f1, <-
Cross references: |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--4.6, 1.4, 0.9, 0.55; HindIII--6.8, 0.65; EcoRI--5.4, 1.9. The insert contains the following restricition sites (approximate kb from the 5' end): HindIII--0.38, 1.02; PstI--0.44; NcoI--0.95; HincII--1.31. Recommendation for verification: PvuII; HindIII; PstI; NcoI; HincII. |
| References |
Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778
Yamamoto R, et al. Cloning and expression of a cDNA encoding human sterol carrier protein 2. Proc. Natl. Acad. Sci. USA 88: 463-467, 1991. PubMed: 1703300
Cremers FP, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene. J. Biol. Chem. 269: 2111-2117, 1994. PubMed: 8294464
Cremers FP, et al. An autosomal homologue of the choroideremia gene colocalizes with the usher syndorme type II locus on the distal part of chromosome 1q. Hum. Mol. Genet. 1: 71-75, 1992. PubMed: 1301160
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