Total size of construct (kb): 17.135
Marker: ampR
Vector: pAT153
Vector type: cloning vector
Insert site: BamHI
Vector size (kb): 3.658
Markers: ampR
Suggested primers for sequencing that flank BamHI site:
Primer1 (forward): 5’-CCTGCTCGCTTCGCTACTTG-3’
Primer2 (reverse): 5’-AGGCGCCAGCAACCGCACCT-3’
Insert
Insert size (kb): 13.477
Insert ends: BamHI
DNA: partial genomic DNA
Source: Molluscum contagiosum virus (MCV)
Genbank accession: U60315, partial
Nucleotides 1-13477 of the insert correspond to nucleotides 166008-179485 of Genbank U60315
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed as 200 ng purified plasmid DNA
References
Bugert JJ, Darai G. Stability of molluscum contagiosum virus DNA among 184 patient isolates: evidence for variability of sequences in the terminal inverted repeats. J. Med. Virol. 33(3): 211-217, 1991. PubMed: 1880497
Melquiot NV, Bugert JJ. Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens. Methods Mol. Biol. 269: 371-384, 2004. PubMed: 15114026
Senkevich TG, et al. Genome sequence of a human tumorigenic poxvirus: prediction of specific host response-evasion genes. Science 273(5276): 813-816, 1996. PubMed: 8670425
