| Vector Information |
Size (kb): 5.2750000953674320 Vector: pBSL193 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,tetR Construct size (kb): 5.275000095367432 Features: marker(s): ampR
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: KpnI...SacI MluI
MCS: MluI KpnI...SacI
coding sequence: tetR |
| Comments |
Restriction digests of the clone give the following sizes (kb):BamHI--3.0, 2.3; EcoRI--3.0, 1.45, 0.6, 0.4; KpnI--3.0, 2.3; MluI--3.15, 2.2; SstI--3.0, 2.35. Plasmid useful for generation of marked deletions and insertions in cloned DNA in vitro. Contains an omega - Tc element flanked by multiple cloning sites duplicated in tandem orientation. Corresponds to the EcoRI/StyI fragment of pBR322, with BamHI, SalI, AccI, HincII, and HindIII sites removed. |
