Restriction digests analyzed on agarose gels give the following sizes (kb): HindIII--7.7, 4.4; XhoI--12.1; BamHI--6.2, 4.7, 1.3. Ap(r). Virus clone biologically active on DNA transfection, rescued virus induces osteosarcomas. Infected D55 cells obtained from ATCC. Constructed by inserting EcoRI digested FBR-MSU integrated pro-viral DNA from non-producer cells Ap(r). Virus clone biologically active on DNA transfection, rescued virus induces osteosarcomas. Biologically active; upon transfection rescued virus induces osteosarcomas. The p75 gag-fos fusion protein is a nuclear phosphoprotein, antigenically related to p12gag, p15gag and p30gag as well as v-fos. This clone was constructed by inserting EcoRI-digested FBR-MSV integrated proviral DNA from non-producer cells into the EcoRI site of Charon 30; the HindIII fragment was then subcloned into the HindIII of pBR322. |
