| 產品名稱 |
pUC8.2-14 |
| 商品貨號 |
B192950 |
| Designations |
pUC8.2-14 |
| GenBank Number |
M38352
|
| Species |
Rhizopus oryzae Went et Prinsen Geerligs |
| Depositors |
MJ Haas, TR Berka, MJ Haas |
| Applications |
produces protein lipase |
| Vector |
Construct size (kb): 4.0 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.286999940872192 Tissue: Rhizopus (delemar) oryzae Gene product: lipase [LIP] Target Gene: lipase |
| Insert Size (kb) |
1.287 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.3; HindIII--3.7, 0.43; KpnI--4.0. The insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.69, 1.02, 1.20; HindIII--0.86; KpnI--0.66; NcoI--0.30. Expression is regulated by lac sequences. Contains the complete coding sequence (nt 46-1224). |
| References |
Berka TR, Haas MJ. Genetically engineered microorganisms containing a gene segment coding for a lipase from Rhizopus delemar. US Patent 5,219,753 dated Jun 15 1993
Haas MJ, et al. Cloning, expression, and characterization of a cDNA encoding a lipase from Rhizopus delemar. Gene 109: 107-113, 1991. PubMed: 1756969
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
