Restriction digests of the clone give the following sizes (kb): BamHI--4.3; EcoRI--3.0, 1.2; NcoI--4.3; PstI--4.3; PvuII--2.6, 1.6. The insert contains the following restriction sites (approximate kb from the T3 primer end): BclI--0.09; NcoI--0.27. Based on the insert size and the alignment with GenBank accession M35266 (1458 nt, CDS 190-792), this clone appears to contains part of the coding sequence and extends into the 3' untranslated region. Detects a 2.1 kb transcript in mouse heart, kidney, liver and testes. |
