A general purpose replicating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Clarke L, Carbon J. Isolation of the centromere-linked CDC10 gene by complementation in yeast. Proc. Natl. Acad. Sci. USA 77: 2173-2177, 1980. PubMed: 6990421
