Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--5.9, 3.0; EcoRI--9.0; HindIII--9.4; BamHI--9.4; PstI--6.3, 1.25, 1.05, 0.92. The GM1416 cell line was incorrectly described as 49,XXXXY. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. The distance between the EcoRI (F5.53, ATCC 61108) and the MspI (H5.52, ATCC 61106) is 48 kb. This probe was derived from C5.5 by digesting with HindIII and religating. This is from the more centromeric end of C5.5. This probe was derived from the cosmid C5.5. C5.5 was isolated from a library constructed by digesting the vector C2RB with BamHI and SmaI and inserting size-selected fragments from GM1416 partially digested with MboI. |
