| 產(chǎn)品名稱 | M158 |
|---|---|
| 商品貨號 | B193738 |
| Organism | Mus musculus, mouse |
| Tissue | breast; mammary gland |
| Cell Type | epithelial cell |
| Product Format | frozen |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | breast cancer |
| Applications | The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene. |
| Clinical Data | female |
| Genes Expressed | Myc/c-Myc, expressed |
| Tumorigenic | yes |
| Comments | The M158 cell line was derived from the mammary tumor of a transgenic mouse carrying the MMTV-c-myc transgene.
c-Myc plays a critical role in activating the transcription of growth-related genes. Elevated expression of c-Myc has been found in almost one-third of breast and colon carcinomas. The cell line M158 overexpresses c-Myc. It is can be used to study the molecular mechanism and function of c-Myc in mammary gland tumors. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium renewal: Every 2 to 3 days. |
| Cryopreservation | Freeze medium: complete growth medium supplemented with an additional 10% bovine calf serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor | P Leder |
| References | Stewart TA, et al. Spontaneous mammary adenocarcinomas in transgenic mice that carry and express MTV/myc fusion genes. Cell 38: 627-637, 1984 PubMed: 6488314 Elson A, et al. Protein-tyrosine phosphatase epsilon. An isoform specifically expressed in mouse mammary tumors initiated by v-Ha-ras OR neu. J. Biol. Chem. 270(44): 26116-26122, 1995 PubMed: 7592814 Amundadottir LT, et al. Signal transduction pathways activated and required for mammary carcinogenesis in response to specific oncogenes. Oncogene 16(6): 737-746, 1998 PubMed:9488037 Fantin VR, et al. A novel mitochondriotoxic small molecule that selectively inhibits tumor cell growth. Cancer Cell 2(1): 29-42, 2002 PubMed: 12150823 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
