| 產品名稱 |
Human herpesvirus 1 |
| 商品貨號 |
B193775 |
| Classification |
Herpesviridae, Simplexvirus |
| Deposited As |
Herpes simplex type 1 |
| Agent |
Human herpesvirus 1 |
| Strain |
KOS |
| Common Name |
Herpes simplex virus 1 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
VR-1493™ was derived from VR-1487™ following treatment with a mycoplasma removal agent to remove mycoplasma contamination. Antigenically cross-reactive with Human herpesvirus 2. |
| Effect on Host |
CPE, syncytial formation, cell rounding, clumping, and sloughing |
| Recommended Host |
Vero (ATCC® CCL-81™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 1-3 days |
| Name of Depositor |
PA Schaffer, University of Pennsylvania |
| Special Collection |
NCRR Contract |
| Source |
Lip lesion of human with cold sore |
| References |
Smith KO. Relationship between the envelope and the infectivity of herpes simplex virus. Proc. Soc. Exp. Biol. Med. 115: 814-816, 1964. PubMed: 14155835
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