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pSV3-dhfr plasmid in <em>Escherichia coli</em>
pSV3-dhfr plasmid in <em>Escherichia coli</em>
規(guī)格:
貨期:
編號:B193924
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pSV3-dhfr plasmid in Escherichia coli
商品貨號 B193924
Designations pSV3-dhfr plasmid in Escherichia coli
Depositors P Berg, Stanford University Medical Center
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Construct size (kb): 8.4
Vector: pSV3-dhfr (plasmid)
Construction: pBR322, SV40, mouse

Features:
marker(s): ampR, mtxR
other: SV40 large T antigen
promoter: SV40
replicon: pMB1
Applications
Expression vector
Shuttle vector
Comments
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture cells.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped freeze-dried
Shipping Information Plasmid in E. coli
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479