| Vector |
Construct size (kb): 5.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PYCR1
Genomic copy number: unique
Gene name: pyrroline-5-carboxylate reductase 1
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.0
Cross references: DNA Seq. Acc.: M77836
Nucleotides 46-1842 of the insert correspond to
nucleotides 1-1792 of M77836 with 99% identity.
DNA Seq. Acc.: T07966; EST: EST05857; Primer: M13-21; THC
id: THC170714
DNA Seq. Acc.: T07967; EST: EST05858; Primer: M13 Reverse;
THC id: THC170714 Insert lengths(kb): 2.0 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: pyrroline-5-carboxylate reductase 1( 1-pyrroline-5-carboxylate reductase, P5C reductase) [PYCR1] |
