Nucleotide (GenBank) :
VB0008
pJSC73 cloning vector from pBR325 and pAT153 for blunt-end cloning.
Biosafety Level
1
Vector Information
Size (kb): 3.5599999427795410
Vector: pJSC73 (plasmid)
Construction: pBR325, pAT153
Marker(s):ampR,cmlR
Construct size (kb): 3.559999942779541
Features: marker(s): ampR, cmlR replicon: pMB1
Comments
Small plasmid vector for cloning of blunt-end DNA fragments (HincII in ampR and PvuII in cmlR).
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Cordingley JS, et al. Clone banks of cDNA from the parasite Schistosoma mansoni: isolation of clones containing a potentially immunodiagnostic antigen gene. Gene 26: 25-39, 1983. PubMed: 6323252
