| Insert |
DNA: genomic Insert lengths(kb): 17.0 Tissue: liver Gene product: DNA Segment, single copy [D12S26] Alleles: A1, A4, A6, B1, B5, A2, A3, B2, B3, B4, C1, C2, C3, C5, A5, C4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 7.6, 7.0, 4.9, 4.34, 3.9; EcoRI--20.0, 11.0, 9.4, 4.7, 3.2; HindIII--20.0, 13.0, 5.4 (doublet), 2.2; XbaI--30.0, 15.0, 0.87; KpnI--20.0, 17.0, 3.3 (doublet), 1.5; SstI--19.0, 8.2, 3.6, 3.0 (doublet), 2.1, 1.9, 1.8, 1.1, 0.87. The EcoRI polymorphisms have a combined PIC value of 0.45 and a combined heterozygosity of 48%. Enzyme(s) not detecting polymorphism: AluI, HaeIII, MspI, RsaI (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
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