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| 產品名稱 | Crithidia luciliae thermophila Roitman et al. |
|---|---|
| 商品貨號 | B194460 |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation | Zelus leucogrammas, Goiania, Brazil, 1977 |
| Product Format | frozen |
| Type Strain | yes |
| Comments | Growth at high temperature Trypanosomatids from fruit Riboprinting and taxonomy Thermal regulation of oxygen-scavenging enzymes Monoclonal antibodies for identification endonuclease-generated fragments of K-DNA, esterase isoenzymes, surface proteins for species identification Multiple distinct site-specific elements in miniexon arrays Cyclopropane fatty acid effect of temperature and osmolarity on growth |
| Medium | ATCC® Medium 355: Crithidia medium |
| Growth Conditions | Max Temperature: 37.0°C Min Temperature: 25.0°C Growth condition: Axenic. Consult product sheet for protocol. |
| Subcultivation | Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Cryopreservation | 1. ? Prepare a 10% (v/v) sterile DMSO solution in fresh ATCC Medium 355.? 2.?? Transfer a culture at peak density to centrifuge tubes and centrifuge at 525 x g for 5 minutes. 3.?? Remove the supernatant and resuspend the cells in ATCC medium 355 to a concentration of 2 x 106 to 2 x 107 cells/ml. 4.?? Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be between 106 and 107 cells/ml and 5% (v/v) DMSO. 5.?? Distribute the cell suspension in 0.5 ml aliquots into 1.0-2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).? The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no longer than 30 min. 6.?? Place the vials in a controlled rate freezing unit.? From room temperature cool at -1°C/min to -40°C.? If the freezing unit can compensate for the heat of fusion, maintain rate at??????? -1°C/min through the heat of fusion.? At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.? Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.? (The cooling rate in this apparatus is approximately???????????? -1°C/min.) ? 7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen freezer. 8.?? To establish a culture from the frozen state place an ampule in a water bath set at 35°C (2-3 min). Immerse the vial just sufficient to cover the frozen material. Do not agitate the vial. 9.?? Immediately after thawing, aseptically remove the contents of the ampule and inoculate into 5 ml of fresh ATCC medium 355 in a 16 x 125 mm screw-capped test tube. Incubate upright at 25°C with caps screwed on tightly. |
| Name of Depositor | I Roitman |
| Chain of Custody | ATCC <<--I Roitman<<--M. Deane <<--- A.L.M. Carvalho |
| Year of Origin | 1977 |
| References | Roitman. Growth of Crithidia at high temperature: Crithidia hutneri sp. n. and Crithidia luciliae thermophila s. sp. n.. J. Protozool. 24: 553-556, 1977. Conchon I, et al. Trypanosomatids, other than Phytomonas spp., isolated and cultured from fruit. J. Protozool. 36: 412-414, 1989. Gannon JT, Linke HA. Growth studies on xenic cultures of Entamoeba gingivalis using established media. Int. J. Parasitol. 19: 835-838, 1989. PubMed: 2635159 Clark CG. Riboprinting: A tool for the study of genetic diversity in microorganisms. J. Eukaryot. Microbiol. 44: 277-283, 1997. PubMed: 9225441 Emtage MA, Bremner TA. Thermal regualtion of active oxygen-scavenging enzymes in Crithidia Luciliae thermophila. J. Parasitol. 79: 809-814, 1993. Goncanlves De Lima VM, et al. Comparison of six isoenzymes from 10 species of Crithidia. J. Protozool. 29: 397-401, 1982. Teixeira MM, Camargo EP. Monoclonal antibodies for the identification of trypanosomatids of the genus Phytomonas. J. Protozool. 36: 262-264, 1989. Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247 Teng SC, et al. A new non-LTR retrotransposon provides evidence for multiple distinct site-specific elements in Crithidia fasciculata miniexon arrays. Nucleic Acids Res. 23: 2929-2936, 1995. PubMed: 7659515 Camargo EP, et al. Electrophoretic analysis of endonuclease-generated fragments of k-DNA, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. J. Protozool. 29: 251-258, 1982. PubMed: 6284925 Da Silva JB, Roitman I. Effect of temperature and osmolarity on growth of Crithidia fasciculata, Crithidia hutneri, Crithidia thermophila, and Herpetomonas samuelpessoai. J. Eukaryot. Microbiol. 29: 269-272, 1982. Cho J, Eichinger D. Crithidia fasciculata induces encystation of Entamoeba invadens in a galactose-dependent manner. J. Parasitol. 84: 705-710, 1998. PubMed: 9714198 type strain |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
