Converts externally fed N penicillin into a cephalosporin-type antibiotic
Converts penicillin G (benzylpenicillin) into deacetoxycephalosporin G
Isolation
N-methyl-n'-nitro-n-nitrosoguanidine (NTG)-derived mutant of ATCC 27064
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
2°C to 8°C
Preceptrol®
no
Type Strain
no
Comments
Mutant decreased in cephalosporin production
Medium
ATCC® Medium 196: Yeast malt extract agar
ATCC® Medium 1877: ISP Medium 1
Growth Conditions
Temperature: 28.0°C
Atmosphere: Aerobic
Name of Depositor
A Demain
Chain of Custody
ATCC <<--A Demain<<--B. Mahro
References
Mahro B, Demain AL. In vivo conversion of penicillin N into a cephalosporin type antibiotic by a non-producing mutant of Streptomyces clavuligerus. Appl. Microbiol. Biotechnol. 27: 272-275, 1987.
Cho H, et al. Elucidation of conditions allowing conversion of penicillin G and other penicillins to deacetoxycephalosporins by resting cells and extracts of Streptomyces clavuligerus NP1. Proc. Natl. Acad. Sci. USA 95: 11544-11548, 1998. PubMed: 9751702
