| 產品名稱 |
pIJ43 [MCB1023] |
| 商品貨號 |
B194683 |
| Designations |
pIJ43 [MCB1023] |
| GenBank Number |
M11200
|
| Species |
Saccharopolyspora erythraea (Waksman) Labeda |
| Depositors |
Eli Lilly & Co., SD Hatch, Eli Lilly & Co. |
| Applications |
contains easily purifiable cassette(s) for construction N-6-amino adenine-N-methyl transferase in another host, produces protein N-6-amino adenine-N-methyl transferase |
| Vector |
Construct size (kb): 16.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: SLP1.2
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available): |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Saccharopolyspora erythraea
Gene symbol: ermE
Gene name: N-6-amino adenine-N-methyl transferase
Contains complete coding sequence?: Y
Strain: NRRL 2338
Type of DNA: genomic
Insert 5' end: BamHI
Insert 3' end: KpnI
Insert size (kb): 1.36
Cross references: DNA Seq. Acc.: M11200
Nucleotides 1-1362 of the insert correspond to
nucleotides 275-1636 of M11200. Insert lengths(kb): 1.360000014305115 Gene product: N-6-amino adenine-N-methyl transferase [ermE] Target Gene: N-6-amino adenine-N-methyl transferase |
| Insert Size (kb) |
1.360 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/KpnI--15, 1.36; EcoRI--8.5, 7.5; BamHI--16. Plasmid contains the following restriction sites (approximate kb from the BamHI site): BamHI--0.0; BglII--15.8; EcoRI--3.9, 12.4; HindIII--0.3, 3.3, 7.3, 12.5; KpnI--1.4, 2.6; PstI--2.2, 11.7; SalI--0.6, 2.5, 8.8, 14.5, 15.7. The insert is useful for transfer to cloning vectors to confer erythromycin resistance in Streptomyces and related hosts. |
| References |
Uchiyama H, Weisblum B. N-methyl transferase of Streptomyces erythraeus that confers resistance to the macrolide-lincosamide-streptogramin B antibiotics: amino acid sequence and its homology to cognate R-factor enzymes from pathogenic bacilli and cocci. Gene 38: 103-110, 1985. PubMed: 3934045
Malin NE, et al. Vectors for cloning in streptomyces. US Patent 4,468,462 dated Aug 28 1984
Fayerman JT, Richardson MA. Cloning vectors for use in streptomyces and related organisms. US Patent 4,513,086 dated Apr 23 1985
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
