| Insert |
DNA: cDNA Insert lengths(kb): 1.100000023841858 Tissue: mast cell KU812 cell line Gene product: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide( Fc fragment of Ige, high affinity I, receptor for, alpha polypeptide, immunoglobulin E receptor, high-affinity, of mast cells, alpha polypeptide, Fc IgE receptor, alpha polypeptide) [FCE1A] Alleles: A2, A2, A1, A1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI/SacI--2.9, 1.1; EcoRI--4.0; HindIII--4.0; XbaI--4.0; SacI--4.0. pGEM-3.110B-1 has been deposited in the NIH repository (ATCC 61328) and to support a patent application (ATCC 67566). This clone encodes a complete alpha chain and includes 5' and 3' flanking sequences with a poly(A) tail. The XbaI site is at the 5' end. The probe used to detect the RFLP was a 0.9 kb XbaI/SfaN1 fragment which includes the entire reading frame but excludes some of the 3' sequences. The entire cDNA was also used. The probe detects human EcoRI fragments of 4.4, 3.0, and 1.5 kb and mouse EcoRI fragments of 4.9, 3.9, and 1.7 kb. Enzyme(s) not detecting polymorphism: AccI, AvaII, BamHI, BanI, BglII, DdeI, EcoRI, EcoRV, HincII, HindIII, HinfI, MspI, PstI, PvuII, SspI, TaqI, XbaI. |
| References |
Tepler I, et al. The gene for the human mast cell high-affinity IgE receptor alpha chain: chromosomal localization to 1q21-q23 and RFLP analysis. Am. J. Hum. Genet. 45: 761-765, 1989. PubMed: 2573277
Shimizu A, et al. Human and rat mast cell high-affinity immunoglobulin E receptors: characterization of putative alpha-chain gene products. Proc. Natl. Acad. Sci. USA 85: 1907-1911, 1988. PubMed: 2964640
Leder P, et al. DNA encoding IGE receptor alpha-subunit or fragment thereof. US Patent 4,962,035 dated Oct 9 1990
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