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Construct size (kb): 6.769999980926514

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS-
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI closer to SalI
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ITGA4
Gene name: integrin, alpha 4 (CD49D, alpha 4 subunit of VLA-4
receptor)
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2 q31-q32; 2, 913.240000 cR
Tissue: HPB-mLT cell line
Disease: leukemia, T-cell
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.805
Cross references: DNA Seq. Acc.: X16983
Nucleotides 1-3805 of the insert correspond to
nucleotides 1-3805 of X16983.

Insert lengths(kb): 3.805000066757202

Tissue: HPB-MLT cell line leukemia, T-cell

Disease: leukemia, T-cell

Gene product: integrin, alpha 4 (CD49D, alpha 4 subunit of VLA-4 receptor)(integrin, alpha 4) [ITGA4]

This is the purified plasmid from ATCC#95494.

Restriction digests of the clone give the following sizes (kb): SalI/XbaI--3.8, 2.9; BamHI--3.3, 3.3; HindIII--5.6, 1.0; EcoRI--3.0, 2.0, 1.8; XbaI--6.7.

The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.83; HindIII--0.94 EcoRI--1.83.