Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
Comments
The early passage history of this item is unknown.
Effect on Host
CPE, massive syncytia formation and sloughing
Recommended Host
HEp-2 (ATCC® CCL-23)
Growth Conditions
Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-7 days
Name of Depositor
RM Chanock, J Crowe, National Institute of Allergy and Infectious Diseases; ATCC?
Special Collection
ATCC
Source
Respiratory secretions from child with acute respiratory disease seen at Children’s Hospital of the District of Columbia, Washington, DC, 1962. Treated ATCC® VR-1401™ to remove mycoplasma at ATCC®.
References
Coates HV, et al. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am. J. Epidemiol. 83: 299-313, 1966. PubMed: 5933417