The cleavage position in the reading frame for cloning sites is (where 3 = between triplets): EcoRI-1; SacI-2; KpnI-2; SmaI-1; BamHI-1; XbaI-1; SalI-1; PstI-2; SphI-2; HindIII-1. The SacI site is not unique. Restriction digests of the clone give the following sizes (kb): EcoRI--8.0; BamHI--8.0; SalI--8.0; PstI--8.0. One of 3 promoter-cloning, YE type shuttle vectors (ATCC 37731 - 37733) with URA3 selection in Saccharomyces cerevisiae, a beta-galactosidase reporter gene and multiple cloning sites differing in reading frame. The sequence and reading frame of the multiple cloning sequence is: 5'GAA TTC GAG CTC GGT ACC CGG GGA TCC TCT AGA GTC GAC CTG CAG GCA TGC AAG CTT GCT CCC 3', from nucleotide 1 of the MCS through CCC for amino acid 8 of beta-galactosidase. |
