| 產品名稱 |
pCP2-4-10 |
| 商品貨號 |
B195363 |
| Designations |
pCP2-4-10 |
| Depositors |
E.I. du Pont, SC Falco, E.I. du Pont |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli HB101 |
| Vector Information |
Size (kb): 0E-16 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Vector end: ClaI
Vector end: HindIII
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
Cross references: Vector: pCP2-4-10 (plasmid) Construction: YEp24, Saccharomyces cerevisiae ILV2 Marker(s):URA3,ampR,ILV2 Construct size (kb): 0.0 Features: marker(s): ILV2
marker(s): URA3
marker(s): ampR
replicon: 2 micron
replicon: pMB1 |
| Applications |
YE-type (episomal) shuttle vector contains sequence acetohydroxyacid synthase shuttle vector |
| Comments |
An episomal shuttle vector with dominant selectable marker for resistance in Saccharomyces cerevisiae to sulfometuron methyl, a sulfonylurea herbicide. The ILV2 fragment can be excised as a ClaI/HindIII fragment. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Falco SC. Selectable markers for yeast transformation. US Patent 4,626,505 dated Dec 2 1986
Sauer BL. Site-specific recombination of DNA in eukaryotic cells. US Patent 4,959,317 dated Sep 25 1990
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
