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in another host, produces protein mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1)

Construct size (kb): 13.80000019073486

DNA: genomic

Insert lengths(kb): 6.0

Gene product: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1) [ATP13]

Target Gene: mitochondrial protein, 43 kDa, required for expression of subunit 9 of F0 ATPase (oli1)

Restriction digests of the clone give the following sizes (kb): EcoRI--6.6, 3.7, 2.3, 1.0, 0.6; SstI--12.0, 1.7; SmaI/XhoI--9.4, 4.6; Xba--5.2, 4.8, 4.4; SalI/SmaI--7.0 (doublet).

The insert contains the following restriction sites (approximate kb from the end closer to the SalI site of the vector [nt of published sequence]): XbaI--0.5, 4.5; HindIII--1.3 [1], 2.3 [1004], 2.5 [1269]; XhoI--1.9 [656]; SstI--0.6, 2.25 [969].

EcoRI--2.5 [1227], 3.2, 4.1.