| Comments |
Restriction digests of the clone give the following sizes (kb): AvaI--3.2, 2.6; EcoRI--4.6, 0.8, 0.64; HindIII--5.0, 0.78. Insert contains the following restriction sites (approximate kb from the 5' end): AvaI--0.15; EcoRI--0.75; HindIII--0.95. Recommendation for verification: EcoRI, HindIII, AvaI. |