| 產(chǎn)品名稱 |
Human rhinovirus 17 |
| 商品貨號(hào) |
B195479 |
| Classification |
Picornaviridae, Enterovirus, Rhinovirus B |
| Agent |
Human rhinovirus 17 |
| Strain |
33342 |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Genome Sequenced Strain |
Human rhinovirus 17, complete genome
|
| Comments |
ATCC® VR-1663™ was treated with diethyl ether at ATCC® to remove Mycoplasma. The previous item number was ATCC® VR-1127™ (NIAID Research Reference Reagent V-106-003-021). Strain 33342 is the prototype strain for Human rhinovirus 17. |
| Effect on Host |
CPE, rounding, sloughing |
| Recommended Host |
H1HeLa (ATCC® CRL-1958™) |
| Growth Conditions |
Temperature: 33°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and then wash monolayer with PBS or serum free medium prior to inoculation. Inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb for 1 hour at 33°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 3 days at 33°C in a humidified 5% CO2 atmosphere while rocking, until CPE is progressed throughout |
| Name of Depositor |
NIAID, ATCC |
| Source |
Presumed from throat swab from adult with upper respiratory illness, North Carolina, 1959 |
| References |
Fenters JD, et al. Propagation of Rhinovirus on WI-38 cell monolayers in rolling bottles. Appl. Microbiol. 15: 1460-1464, 1967.
Tapparel C, et al. New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features. BMC Genomics 8: 224, 2007. PubMed: 17623054
|
| Cross References |
Nucleotide (GenBank) :
EF173420.1
Human rhinovirus 17, complete genome
|
