IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A Saccharomyces cerevisiae/Escherichia coli cosmid shuttle vector.
Media
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References
Morris DW, et al. Plasmid vectors capable of transferring large DNA fragments to yeast. DNA 1: 27-36, 1981. PubMed: 6299664
