Restriction digests of the clone give the following sizes (kb): BamHI--3.2; EcoRI--2.7, 0.55; PstI--3.1, 0.1; PvuII--2.5, 0.3 (doublet); XbaI--2.8, 0.4. The insert contains the following restriction sites (nt from the 5' end): PstI--55; XbaI--366. Detects rat genomic restriction fragments of the following sizes (kb): HindIII--greater than 9.0 (SPI2.3), 5.0, 1.0 (SPI2.2); 1.0 (SPI2.1); BamHI--greater than 8.0 (SPI2.3); 2.6, 2.5 (SPI2.2); greater than 6.0 (SPI2.1). Contains sequences highly conserved among SPI2.1, SPI2.2, and SPI2.3. Detects a mRNA of 1.8 kb. |
