Restriction digests of the clone give the following sizes (kb): EcoRI--5.7 (doublet), 4.4; HindIII--5.6, 5.0, 3.8, 0.75 (doublet); EcoRI/HindIII--4.4, 3.8 (doublet), 1.2 (doublet), 0.75 (doublet); BamHI--5.4, 5.0, 4.2, 0.65 (doublet); PvuII--7.8, 5.8, 2.6. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.7, 5.0. Detects a human genomic EcoRI fragment of 6.2 kb, The 0.7 kb HindIII/EcoRI fragment contains coding sequences. and a mRNA of 6.8 kb in HeLa cells. The plasmid contains two copies of the insert. |
