| 產品名稱 |
pB21BSM |
| 商品貨號 |
B196396 |
| Designations |
pB21BSM |
| Species |
Beet necrotic yellow vein virus |
| Depositors |
KE Richards |
| Vector |
Construct size (kb): 4.670000076293945 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.460000038146973 Gene product: genome, partial |
| Insert Size (kb) |
1.460 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindII--3.1, 1.5; EcoRI--4.6; HindIII--4.6. To synthesize antisense RNA in vitro, cut with HindIII and transcribe from vector T7 promoter. The insert is nt 2324-3789 of RNA-2 of beet necrotic yellow vein virus isolate F3 from Chenopodium quinoa and is noninfectious. |
| Classification |
Furovirus |
| References |
Bouzoubaa S, et al. Nucleotide sequence of beet necrotic yellow vein virus RNA-2. J. Gen. Virol. 67: 1689-1700, 1986.
Ken E Richards, personal communication
|
