Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following sites separated by (kb) (approximately): SacI- 0.3- KpnI- 1.3- KpnI- 1.0- BamHI- 1.2- XbaI- 0.8- BamHI- 0.4- PstI- 0.4- XbaI- 0.4- BamHI- 0.4- PstI- 0.4- KpnI- 0.5- SacI.
Carries plasmid pG72/T1 with wild-type gene
MSM1 is the nuclear gene encoding the mitochondrial methionyl-tRNA synthetase.
The smallest region capable of complementing a msm1-1 mutation is defined by a KpnI-PstI fragment of approximately 3 kb from the middle of the insert, and spanning the BamHI and XbaI sites separated by 1.2 kb.
The probe, pG72/T1, has been deposited in Escherichia coli RR1 as ATCC 37663 and in Saccharomyces cerevisiae C122/U3 as ATCC 66319.
Tzagoloff A, et al. Characterization of MSM1, the structural gene for yeast mitochondrial methionyl-tRNA synthetase. Eur. J. Biochem. 179: 365-371, 1989. PubMed: 2645139
