| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--6.6, 5.0, 3.1, 3.0, 2.7 (doublet), 2.3, 1.9, 1.8 (doublet), 1.3, 1.2, 1.0 (doublet), 0.9 (doublet), 0.85, 0.8 (doublet), 0.75, 0.55 (doublet), 0.4. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 6.732, 1.006 (vector); 5.10, 3.165, 2.942, 2.79, 2.648, 2.34, 1.892, 1.825, 1.766, 1.277, 1.141, 1.059 (2), 0.917 (2), 0.873, 0.814, 0.771, 0.767, 0.712, 0.526, 0.485, 0.381. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 139499-177141 of the sequence of chromosome VIII. |
