| 產品名稱 |
LORIST6 |
| 商品貨號 |
B196683 |
| Designations |
LORIST6 |
| Depositors |
TJ Gibson |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 5.1599998474121090 Vector: LORIST6 (cosmid) Promoters: Promoter T7 Marker(s):kanR,neoR Construct size (kb): 5.159999847412109 Features: marker(s): kanR, neoR
promoter: SP6, T7
replicon: lambda
terminator: trpA rrnC |
| Applications |
vector for constructing genomic libraries vector for other uses vector permitting RNA synthesis in vitro |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--5.7; HindIII--5.7. Accepts blunt-ended DNA. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector for genomic library construction. Insert-end-specific probes may be generated from T7 and SP6 promoters. |
| Media |
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
|
| Growth Conditions |
Temperature: 30.0°C |
| References |
Gibson TJ, et al. Lorist6, a cosmid vector with BamHI, NotI, ScaI and HindIII cloning sites and altered neomycin phosphotransferase gene expression. Gene 53: 283-286, 1987. PubMed: 3038694
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
