Restriction digests of the clone give the following sizes (kb): BamHI--9.6; SalI--9.6. G418 resistance (neo) is expressed under the control of the Drosophila hsp70 promoter. There are no pBR322 sequences in the vector. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Cosmid vector for chromosome walking and P-element-mediated germline transformation in Drosophila. |
