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Distribution host: Escherichia coli HB101 (ATCC 33694)

Size (kb): 4.6430001258850100

DESCRIPTION OF VECTOR:
Intact vector size: 4.643
Type of vector: plasmid
Cloning sites: HindIII PstI SalI BamHI ClaI SmaI KpnI SacI EcoRI
Polylinker sites: HindIII PstI SalI BamHI [tk-promoter luc+ SV40 poly (A)]ClaI
SmaI KpnI SacI EcoRI
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
MCS: HindIII...BamHI, ->, 1-36
promoter: truncated TK promoter, 37-131
reporter group: luciferase (luc+), ->, 170-1822
terminator: SV40 late polyadenylation, 1854-2075
MCS: ClaI...EcoRI, 2078-2106
replicon: pMB1, 2519-2519
marker(s): ampR, <-, 3277-4137
terminator: SV40 large T-antigen polyadenylation, 4269-4640

Vector: pTATALUC+ (plasmid)

Promoters: Promoter truncated TK promoter

Marker(s):ampR

Construct size (kb): 4.64300012588501

Features: marker(s): ampR
promoter: truncated TK promoter
replicon: pMB1
reporter group: luciferase (luc+)
MCS: ClaI...EcoRI
MCS: HindIII...BamHI
terminator: SV40 large T-antigen polyadenylation
terminator: SV40 late polyadenylation

reporter construct

shuttle vector

Restriction digests of the clone give the following sizes (kb): BamHI--4.6; HindIII--4.6; EcoRI--4.6.

The vector contains modified luciferase (luc+) gene from the firefly Photinus pyralis in order to increase the yield of recoverable luciferase activity after transfection and to eliminate potential cryptic regulatory elements.

The promoters driving luc+ transcription in these two vectors represent different 5' deletions of the tk promoter ending at -32 and -105, respectively.

The vector pTATALUC+ carries only the TATA box to avoid interference of the tk-derived CAAT and GC boxes with the regulatory elements of interest.

The optimized luciferase reporter gene vectors (ATCC 87630 - 87633) provide valuable tools for the analysis of eukaryotic regulatory DNA elements.

The vectors pTATALUC+ (ATCC 87631) and ptkLUC+ (ATCC 87632) were designed for the characterization of isolated cis regulatory elements.

Temperature: 37.0°C

component of:ATCC 87654