It is important to check whether the strain is [rho+] or [rho-] prior to use for complementation analyses.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATalpha pet1 arg4 thr1 trp5-48 his5-2 lys1 ade2-1 leu1 gal2 met1 ura3
RefSherman F, Slonimski PP. Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. Biophys. Acta 90: 1-15, 1964. , (Beck J, et al. Autonomy and biogenesis of mitochondria and chloroplasts. : North Holland/American Elsevier; 1971.)
Preceptrol®
no
Mating Type
alpha
Ploidy
haploid
Comments
Respiration: uncharacterized nuclear petite genes (pet) (CE1)
Genetic petites become [rho-] at a high frequency. It is important to check whether the strain is [rho+] or [rho-] prior to use for complementation analyses.
Medium
ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Name of Depositor
YGSC
Special Collection
Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--S. Fogel
References
Sherman F, Slonimski PP. Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. Biophys. Acta 90: 1-15, 1964.
Beck J, et al. Autonomy and biogenesis of mitochondria and chloroplasts. : North Holland/American Elsevier; 1971.
Sherman F, Slonimski PP. Respiration-deficient mutants of yeast. II. Biochemistry. Biochim. Biophys. Acta 90: 1-15, 1964.
Beck J, et al. Autonomy and biogenesis of mitochondria and chloroplasts. : North Holland/American Elsevier; 1971.