Plasmid for use in RK2-oriT-mediated conjugal transfer of gram negative bacterial chromosomes.
Media
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References
Yakobson EA, Guiney DG Jr.. Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5. J. Bacteriol. 160: 451-453, 1984. PubMed: 6090433
