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pCHR81
pCHR81
規(guī)格:
貨期:
編號:B197874
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pCHR81
商品貨號 B197874
Designations pCHR81
Depositors C Sasakawa
Biosafety Level 1
Vector Information
Size (kb): 40.0000000000000000
Vector: pCHR81 (plasmid)
Marker(s):kanR,tmpR
Construct size (kb): 40.0
Features: marker(s): kanR, tmpR
replicon: R388
Applications
vector useful for transposon mutagenesis
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--32, 4.0, 1.8; PstI--36, 2.7, 1.8; HindIII--38, 3.8, 3.0.
Replication is temperature-sensitive.
Can be used as a suicide vector for the delivery of transposable elements.
Constructed by mutagenizing R388 to construct a temperature sensitive derivative and then transposing on Tn5.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Sasakawa C, Yoshikawa M. A series of Tn5 variants with various drug-resistance markers and suicide vector for transposon mutagenesis. Gene 56: 283-288, 1987. PubMed: 2824292

Shipped frozen
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479