| 產品名稱 |
pKB702 |
| 商品貨號 |
B198097 |
| Designations |
pKB702 |
| GenBank Number |
M10431
|
| Species |
Escherichia coli (Migula) Castellani and Chalmers |
| Depositors |
OmniGene, Inc., KC Backman, OmniGene, Inc. |
| Applications |
produces protein chorismate mutase-P-prephenate dehydrogenase deletion variant |
| Vector |
Construct size (kb): 6.900000095367432 |
| Insert |
DNA: genomic Insert lengths(kb): 2.539999961853027 Gene product: 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase [aroF] Target Gene: chorismate mutase-P-prephenate dehydrogenase deletion variant |
| Insert Size (kb) |
2.540 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.5; PstI--4.4, 2.5; EcoRI--5.6, 1.35. Encodes a catalytically functional enzyme (aa 1-337). Deletion of the C-terminal 49 aa desensitizes the enzyme to phenylalanine feedback inhibition. Excision of the insert with HindIII yields a lac promoter - pheA' - aroF cassette. Production of phenylalanine is enhanced when the pheA structural gene is introduced as part of a synthetic operon, rather than as a single deregulated structural gene. |
| Classification |
Enterobacteriaceae, Escherichia |
| References |
Backman KC. Method of biosynthesis and cells therefor. US Patent 4,839,286 dated Jun 13 1989
Backman KC. Enzyme deregulation. US Patent 4,753,883 dated Jun 28 1988
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
