| Insert |
DNA: genomic Insert lengths(kb): 1.700000047683716 Gene product: DNA Segment, single copy [D16S246] Alleles: G1, G2, H1, A1, A2, B1, B2, C1, C2, D1, D2, E1, E2, F1, F2, H2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--4.37, 1.8, 0.5; HindIII--6.8; EcoRI--6.8; BamHI--6.8; HindIII/EcoRI--4.37, 2.3. This probe is localized proximal to CMM65 (D16S84) and distal to the breakpoint in GM2324 t(16;22) (p13.3;q21). Minor variations were detected in the allele fragment sizes, indicating this probe may be located close to a VNTR. Contains two PstI fragments subcloned from cosmid 218. The 1.7 kb fragment detects the RFLP. Also contains some mouse DNA. Enzyme(s) not detecting polymorphism: HindIII, SacI. |