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Size (kb): 5.2699999809265140

Vector: pCu414CUP1 (phagemid)

Promoters: Promoter for expression CUP1

Construction: pRS414, CUP1 promoter

Marker(s):TRP1,ampR

Construct size (kb): 5.269999980926514

Features: marker(s): TRP1
marker(s): ampR
promoter for expression: CUP1
replicon: ARSH4
replicon: f1
replicon: pMB1
MCS: SpeI...XhoI
terminator: CYC1
centromere: CEN6

cloning vector

Restriction digests of the vector give the following sizes (kb): SacI--5.3; SacI/SpeI--5.0, 0.26.

Gene CUP1 is the most potent for copper induction. The CUP1 promoter can be used to creat a series of versatile vectors to allow its utilization to control the expression of heterologous genes in S.cerevisiae.

component of:ATCC 87778