| 產品名稱 |
pJWK20 |
| 商品貨號 |
B198430 |
| Designations |
pJWK20 |
| Species |
Bacillus thuringiensis subsp. kurstaki de Barjac and Lemille |
| Depositors |
The University of Washington, JF Ryan, The University of Washington |
| Applications |
produces protein crystal protein (delta endotoxin) |
| Vector |
Construct size (kb): 20.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: U
Strain: HD-73
Type of DNA: genomic
Insert end: BglII
Insert end: BglII
Insert size (kb): 16.2
Cross references: DESCRIPTION OF INSERT COMPONENT:
Genome: Bacillus thuringiensis subsp. kurstaki
Gene name: crystal protein (delta endotoxin)
Contains complete coding sequence?: U
Strain: HD-73
Type of DNA: genomic
Insert end: BglII
Insert end: BglII
Insert size (kb): 16.2
Cross references: Insert lengths(kb): 16.20000076293945 Gene product: crystal protein (delta endotoxin) Target Gene: crystal protein (delta endotoxin) |
| Insert Size (kb) |
16.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Expressed protein recognized by antibody to crystal protein of strain HD-1. Insert derived from the 45 megadalton plasmid of the source strain. |
| References |
Schnepf HE, Whiteley HR. Cloning and expression of the Bacillus thuringiensis crystal protein. Proc. Natl. Acad. Sci. USA 78: 2893-2897, 1981. PubMed: 7019914
Schmepf HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,467,036 dated Aug 21 1984
Schanegl HE, Whiteley HR. Bacillus thuringiensis crystal protein in Escherichia coli. US Patent 4,448,885 dated May 15 1984
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
